NEWS

Snippertlab-Bruens

Lotte gets her PhD!!!

Much delayed by COVID, but Lotte was finally allowed to defend her thesis. Opponents and guests were virtually present, while Lotte filled the empty room with great display of her expertise and knowledge!

Snippertlab-Maarten

Expansion

Yes, he is different! We welcome the first MD in our group! Maarten will carry out a phase1/2 trial to test a new therapy against KRAS mutant colon cancer. In parallel he will test organoids as companion diagnostics. Maarten will be co-supervised by Jeanine Roodhart, medical oncologist in our hospital.

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Review in Developmental Cell

COVID lockdown provided a window to reexamine current literature regarding stem cell function and environmental control. Joris took the lead and shaped his long-term interest into a new review.

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Filling up the house

Lotte publishes experimental data in Cell reports that the number of functional stem cells increases in intestinal crypts upon caloric restriction, confirming prior studies using marker gene expression.

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Coronavirus: easing lockdown measures

Most of our lab work has been restricted since half of March. After about two months, we are enthusiastic to slowly reopening the labs. Virtual meetings are likely to stay in some sort…

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Metastatic origin in Cell Stem Cell

Thankful for our great and long-lasting collaboration with the Van Rheenen laboratory! New work, spearheaded by the Van Rheenen lab, in Cell Stem Cell about the origin of liver metastases from colorectal cancers. Using genetic mouse models and intravital imaging, it turns out that the major suspects, i.e. so-called cancer stem cells, are not the predominant seeders of metastases.

snippertlab-ENWgroot

NWO groot

Hugo is a member of a multidisciplinary research team (AMOLF, NKI, Hubrecht & UMCU) that successfully applied for a NWO groot research grant (~€2,5M). The team will perform functional studies on rare cell types in the gut to delineate their relationship with common diseases like Crohn’s, obesity, infections or irritable bowel syndrome.

Snippertlab-Trends in Cancer

Review in Trends in Cancer

We wrote a review about the types of oncogenic mutations in the MAPK pathway in colorectal cancer. They are similar but different. Unfortunately, studying them is often comparing apples to oranges. Organoid models and CRISPR genome editing might pave the way…

Snippertlab-MarieCurie

Marie Curie for Michiel

Michiel was awarded a prestigious Marie Curie research grant to continue his science in our lab after an earlier successful period at MSKCC in NY, USA. Well done! Michiel will study genetic alterations in esophageal adenocarcinoma using patient organoids and high-end imaging.

Snippertlab-Post

PhD defense Jasmin Post

Jasmin successfully defended her thesis called ‘Similar but different: Oncogenic MAPK pathway mutations in colorectal cancer’. Jasmin is fully prepared to take on the business world. We wish her all the luck in her career and life.

stem cell niche

Expansion

We welcome two new PhD students to our team, J&J.

As students, Julian and Joris have both been matured in our lab, with their grand finales at Rockefeller University (NY) and University of Cambridge (UK) respectively.

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Health~Holland grant

Proud to be one of the seven projects in the Organ-on-Chip Showcases call that have been awarded a grant by Top Sector Life Sciences & Health (Health~Holland). I will continue to explore the use of microfluidics (from industrial partner VyCAP) with live-cell imaging of organoids to study tumor evolution.

Snippertlab-Oost

PhD defense Koen Oost

Koen successfully defended his thesis called ‘Cell fate dynamics in intestinal homeostasis and cancer’. He is now gone to the lab of Jacco van Rheenen (NKI, Amsterdam) in preparation to his upcoming adventure in the lab of Prisca Liberali (FMI, Basel). We wish him all the luck in science and life.

snippertlab-CIN

Chromosomal instability in Nature Genetics

Our collaborative study with the Kops laboratory is published in Nature Genetics. We performed detailed live-cell microscopy on patient-derived tumor organoids from colorectal cancer patients, to study chromosomal instability levels and cell fate options after erroneous divisions in correlation to karyotype evolution. Among others we show that chromosomal instability is prevalent and ongoing in CRCs, including those designated as hypermutated (microsatellite instable). Great work by Ana and Bas.

Snippertlab-Ovarian cancer

More organoids in Nature Medicine

Fantastic work from the Kloosterman and Clevers laboratories in Nature Medicine about the development of a new platform for the culture of ovarian cancer organoids. Nizar worked hard on the drug screening of these organoids to support the claim that these organoids can assist in personalized therapy design as well as drug development in a pre-clinical setting.

Snippertlab-NF1

New knock-out organoids

Our new study is published in Oncotarget, featuring a CRISPR-screen to test the impact of RASGAP deficiencies on anti-EGFR therapy-resistance in colorectal cancer. Fantastic work by Jasmin to make genetic knock-out organoids and optimize biochemical analysis of the MAPK pathway in these models

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TOP grant

Together with the laboratories of Madelon Maurice and Onno Kranenburg, we received a TOP grant of €675k from ZonMW, the Netherlands Organization for Health Research and Development. We aim to understand why seemingly identical combinations of genetic alterations (mutations) can lead to different forms of colorectal cancer in terms of aggressiveness and therapy-resistance.

2017 nature-crop2

Expansion

We welcome 2 new Postdocs to join our team.
Michiel Boekhout finished his first postdoc at MSKCC in NY, USA, studying double-strand break formation in DNA during meiosis (Keeney lab).
Suzanne van der Horst studied stem cells in C. elegans using real-time imaging and CRISPR-mediated genetic knock-ins (Van den Heuvel lab, UU).

Petra van Leenen finished her bachelors at the Hogeschool Utrecht after a successful internship in our lab. She will stay as a research technician.

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ERC starting grant

Hugo received a prestigious ERC starting grant (€1,5M) from the EU in support of our studies to understand the cause and consequence of diverging behaviors between different tumor cell types. The project will rely heavily on real-time monitoring of cell type specification in tumors (organoids), as well as quantitative measurements of signaling activities at the single-cell level. 

2018 MSB – crop

New paper is out

Integrated multi-omics analyses of organoid model systems to study lineage specification in the intestinal epithelium (Mol Syst Biol). Great work, spearheaded by the lab of Michiel Vermeulen, RIMLS, Nijmegen.

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HFSP young investigator grant

Together with the laboratories of Young Seok Ju (KAIST, Daejeon, SK) and Bon-Kyoung Koo (IMBA, Vienna, Austria), we received a Young Investigator grant ($1M) from the Human Frontier Science Program. We will study hyper mutagenesis on the course of tumor evolution in gastric cancers.

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Launch Oncode Institute

Official launch of Oncode Institute. The institute will support fundamental research in our fight against cancer. Hugo is proud to be a founding scientist of Oncode as junior investigator.

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STAR reporter published in Cell reports

We developed and validated a synthetic enhancer/promoter element that provides specific transcriptional activity in intestinal stem cells. The reporter, called STAR, is a user-friendly system to monitor and manipulate stem cells in human organoids. Among others, we documented that cell fate plasticity in tumors is an inherited trait from normal cells, rather than acquired by specific cancer mutations.

news crypt fusion

Crypt fission is balanced by fusion

Using intravital imaging technology that is developed and performed in the lab of Jacco van Rheenen (NKI), Lotte identified that neighboring crypts (stem cell compartments in the gut) can fuse together. The fusion process looks almost identical to crypt fission (splitting in two) but is in the reverse direction, presumably explaining why fusion has never been picked up before.