Super happy with this technology, foremost as we now can make (complex) knock-ins in human organoids on weekly basis. Major advantages are no off-targets. As a consequence, you can pool all knock-in cells to expedite culture expansion and/or maintenance of polyclonality (in case of tumors). Targeting vectors enable one-step cloning (and all are available @Addgene).

Overall result, amazing looking knock-in organoids within 2wks! @PLOS BIOLOGY

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